• Preeti Upadhyay
• Harshada Joshi

Lactobacillus strains are inhabitant of versatile environmental niche and forms a major part of the probiotics, intestinal microflora and used in the production of fermented dairy products. The objective of the present study was to evaluate the probiotic potential of Lactobacillus rhamnosus CW48 isolated from cow milk. Properties like antibacterial activity, bile tolerance and antibiotic resistance forms the basis for an organism to be a probiotic organism. L. rhamnosus CW48 showed antagonistic activity in cell free supernatant (CSF) against gram-positive organisms like Bacillus subtilis and Bacillus cereus, and gram-negative organisms like Escherichia coli and Pseudomonas putida. In neutralized CSF antibacterial activity was seen against all test organisms except P.putida. When the neutralized CFS was treated with enzyme protease (20mg/ml), zone of inhibition disappeared, confirming the role of bacteriocin protein in antibacterial activity. L. rhamnosus CW48 tolerated bile salt-oxgall up to the concentration of 0.3% (w/v) and also showed resistance to various antibiotics used in present study. L.rhamnosus CW48 possess antibacterial activity, bile tolerance and antibiotic resistance. The isolate can be used as a potential probiotic organism for the benefit of human population by improving health services.

antibacterial activity, antibiotics, bacteriocin, bile tolerance, cow milk, LAB, Lactobacillus rhamnosus, probiotic

"Molecular Characterization and Evaluation of Probiotic Potential of Lactobacillus rhamnosus CW48 isolated from Cow Milk", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 2, page no.627-632, February-2019, Available :http://www.jetir.org/papers/JETIR1902785.pdf

"Molecular Characterization and Evaluation of Probiotic Potential of Lactobacillus rhamnosus CW48 isolated from Cow Milk", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 2, page no. pp627-632, February-2019, Available at : http://www.jetir.org/papers/JETIR1902785.pdf