Abstract
The low and high cost bread samples were purchased from local market, kumbakonam. The high and low cost bread was allowed for spoilage. It was comparative analysis of high cost of bread ingredients and low cost of bread ingredients mainly determined with Energy, Protein, Carbohydrate, Sugar, Fat Saturated fat, Cholesterol, Vitamin, Thiamine, Niacin, Mineral, Calcium, Iron and Sodium were analysed in both sample. Four fungi such as Aspergillus niger, A.flavus, Pencillum sp. and Fusarium sp. were isolated from spoilage bread sample of branded company as well as local company bread analysed. Phytochemicals quantitative and qualitative such as alkaloids, flavonoids, phenols, phlobactins, protein, carbohydrate, saponin, steroids, tannin, and terpenoids were analysed from tested plant. The different concentration 25µl, 50µl, 75µl and 100µl crude extract which inhibit the growth of fungi inhibition condition. According to the biocontrol measures of antifungal activity citrus limon (11.00±3.67mm, 07.00±2.34mm,08.00±2.67mm ,10.00±3.34mm) against bread spoilage fungi was determined. The selected Citrus limon fruit for antifungal properties were excellent performance because of rich quantity of primary and secondary metabolites. The functional group characterized in FTIR analysis of Citrus limon fruit extracts was analysed (3986.16, 3855.40, 3751.47, 3400.72, 2975.95, 29.00.35, 2366.65, 19.24.54, 1648.14, 1389.60, 1332.04, 1263.04, 1050.43, 880.74, 671.38 and 437.53).