• R.Sabina gowri
• Dr.R.Krishnaveni
• Dr.V.Eugin Amala

The infected eye swabs were collected from 20 patients (Male and female) eye infection symptoms who attend the eye clinics and government hospital in kumbakonam. During period from January-February 2018. Then collected samples are transported the microbiology laboratory. The infected eyes sample was serial diluted and inoculated into the nutrient agar medium and incubated. The isolates were gram +cocci, gram -cocci, gram + rods and gram -rod shaped and motile organisms. By biochemical characterization, the isolates was identified as belonging to the following genus.The identification of conjuctival isolate samples it shows The amount 20 samples the highest microbial isolate is is S.aureus it shows 10 members positive (50%), The lower level of microbes isolates is E.coli 2 strains are isolated among 20 persons. The positive result is (10%), keratitis infection samples it shows The amount 20 samples the microbial isolates is S.aureus it shows 9 member positive (45%) The lower level of microbes isolates is E.coli 4 strains are isolated among 20 persons. The positive result is (10%), The identification of microbes on keratitis infection samples it shows The amount 20 samples the microbial isolates is s.pyogenes it shows 11 members positive (55), The lower level of microbes isolates is E.coli 1 strains are isolated among 20 persons. The positive result is (5%).In the present investigation the antibiotic resistant test the staphylococcus aureus, shows the maximum zone of inhibition occurred in Erythromycin (12mm), followed Streptococcus pyogenes, shows the maximum zone of inhibition occurred in penicillin (14mm), Escherichia coli, Ampicillin (12 mm), and Pseudomonas aeruginosa, Ampicillin and Erythromycin (12mm). and Proteus sp - tetracycline and Erythromycin (12 mm). In the present investigations it shows antimicrobial activity of Medicinal plants, The maximum zone of inhibition is (15mm) staphylococcus aureus in Tabernaemontana Divaricata (Acetone with ethanol extract) (17mm), Streptococcus pyogenesin Ocimum sanctum (Acetone with ethanol extract) (20mm), Escherichia coli in Ocimum sanctum (Aceton extract), (15mm) Pseudomonas aeruginosain Lecus aspera (Ethanol extract) and (15mm) Proteus sp in Ricinus communis. Phytochemical screening of individual extracts were qualitatively tested for the presence of various phytochemical constituents namely alkaloids, carboxylic acids, coumarins, flavonoids, phenols, quinones, resins, steroids, fixed oils, saponins, tannins, glycosides In the present investigation the HPLC technique test give some peaks in each particular herbal plant leaf extract , the plants are Leucas aspera, Ocimum sanctum ,Ricinus communis, Tabernaemontana divaricata that result given some phytochemicals components like, Hydroxybenzonic acid, Luteolin, Benzoic acid, Coumeric acid, Rutin, Hdroxy benzoic acid, Gallic acid, Ellagic acid, Ocimene, Germacrene, Coumaric acid, Syringic acid, Ferulic acid, Chlorogenic acid, Catechin, Ethyllinoleate, Hexahydroxyformesyl acetone, Mysristic acid, Apigenin, Quercetin are present in given plant extract. At conclusiongives better zone of inhibition compare to chemical antibiotics, and sensitive to bacterial isolates so, patient may use instead of using chemical antibiotics prefer herbal antibiotics in future.

Tabernaemontana divaricate, Ocimum sanctum, Lecus aspera, Ricinus communis, HPLC, eye infection, E.coli, S.pyogenes, P.aeruginosa, S.aureus, Proteus sp, Phtochemical, Antimicrobial, Antibiotic

"ANALYSING ANTIMICROBIAL ACTIVITY AND PHYTOCHEMICAL STUDY OF MICROBES FROMOPHTHALMIC INFECTION AGAINST OCIMUM SACNTUM. L, LECUS ASPERA. L, TABERNAEMONTANA DIVARICATA. L, RICINUS COMMUNIS. L.", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 2, page no.472-477, February-2019, Available :http://www.jetir.org/papers/JETIR1902B68.pdf

"ANALYSING ANTIMICROBIAL ACTIVITY AND PHYTOCHEMICAL STUDY OF MICROBES FROMOPHTHALMIC INFECTION AGAINST OCIMUM SACNTUM. L, LECUS ASPERA. L, TABERNAEMONTANA DIVARICATA. L, RICINUS COMMUNIS. L.", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 2, page no. pp472-477, February-2019, Available at : http://www.jetir.org/papers/JETIR1902B68.pdf