• Moorthy M
• Anbalagan S
• Sankareswaran M

Cellulose is the initial product of photosynthesis in terrestrial environments and the most abundant renewable bioresource produced in the biosphere. This enzyme is produced by several microorganisms, commonly by bacteria fungi and actiomycetes. The main objective of the study is to explore easy and cost effective method to produce the cellulase enzyme by using sugar cane bagasse, ragistraw and rice straw as substrate, which is an agro waste. Cellulase-producing bacteria were isolated from soil. Among the isolates, three strains showing maximum activity for purified and sub cultured on Carboxymethyl Cellulose (CMC) agar plates. Among the three strains, one strain was given maximum enzyme activity assay was measured by Dinitro Salicylic Acid (DNS) Method. The isolate were identified as Acinetobacter junii by 16S rRNA gene sequence analysis. Optimization of the fermentation medium for maximum cellulase production was carried out via submerged fermentation (SmF) using sugarcane bagasse, rice straw and ragi straw as substrates was studied. The highest production of cellulase was obtained with Acinetobacter junii when 3.0g sugarcane bagasse at 40°C, pH 7.5, 2% Inoculum level in 48 hrs.

Cellulose, Acinetobacter junii, Agro waste, Submerged fermentation.

"OPTIMIZATION OF CELLULASE PRODUCTION BY Acinetobacter junii ISOLATED FROM SOIL", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 2, page no.663-674, February-2019, Available :http://www.jetir.org/papers/JETIR1902B95.pdf

"OPTIMIZATION OF CELLULASE PRODUCTION BY Acinetobacter junii ISOLATED FROM SOIL", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 2, page no. pp663-674, February-2019, Available at : http://www.jetir.org/papers/JETIR1902B95.pdf