• Rekha BabuBhai Bhuriya

We have immobilized urease on copolymer of acryl amide and 2-hydroxy ethyl methaacrylate by covalent coupling .. We have carried out covalent coupling by activating the amino group by glutaraldehyde and hydroxyl group by p-tolyl sulphonyl chloride and p-benzoquinone. We have optimized various covalent coupling conditions such as activation agent concentration, activation agent coupling time, enzyme loading , enzyme coupling time, and coupling pH. We have carried out activation of amino and hydroxyl group separately and have also carried out simultaneous activation of both groups and found some interesting results. We have further carried out the hydrolysis of urea by batch process using covalently bound enzyme.

acrylamide-co-2-hydroxy ethyl methaacrylate,, urease, covalent coupling, urea hydrolysis, batch process.

"Covalent coupling of Urease on Acrylamide-co-2-hydroxy ethyl methaacrylate and Its use in urea hydrolysis by batch process.", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 2, page no.298-304, February-2019, Available :http://www.jetir.org/papers/JETIR1902C50.pdf

"Covalent coupling of Urease on Acrylamide-co-2-hydroxy ethyl methaacrylate and Its use in urea hydrolysis by batch process.", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 2, page no. pp298-304, February-2019, Available at : http://www.jetir.org/papers/JETIR1902C50.pdf