• Urvi K. Bhanushali
• Dr.Reena G. Desai

Lactic acid bacteria are associated with habitats rich with nutrients, such as various dairy products, certain fermented food items as well as plant materials. They have also been found in human intestine where they are said to be inhibiting unwanted gut microorganisms by producing compounds such as organic acids, hydrogen peroxide, diacetyl and bactericidal proteins i.e. Bacteriocin. In the current study Lactic acid bacteria were isolated from Dairy products using MRS medium. All the 16 isolates were screened for production of Bacteriocin against indicator strains available in college laboratory culture collection such as Bacillus subtilis, Escherichia coli, Staphylococcus aureus, Staphylococcus epidermidis, Salmonella paratyphi A. Along with the known indicator strains, the bacteriocin activity was also checked for inhibition against isolated plant pathogen S2 and S3 from infected Solanaceous crops using Kelman's TZC medium(modified). Ten isolates found to be inhibiting the indicator strains were selected further for application of Bacteriocin against isolated plant pathogen. The bacteriocin from U1, U2 and U5 LAB isolates giving the highest inhibition against the isolated plant pathogen S2 and S3 were selected for production of bacteriocin. The produced Bacteriocin from the selected strains were extracted from the cell free supernatant by Ammonium Sulfate precipitation followed by Dialysis. These partially purified bacteriocin can be used in the field to control the bacterial wilt disease without using any chemical control agents and hence the soil pollution by the chemical can be stopped.

Bacteriocin, Lactic acid bacteria, Bacterial wilt, Solanaceae

"Study of application of bacteriocin produced by lactic acid bacteria against isolated plant pathogenic bacteria responsible for bacterial wilt in Solanaceous plants", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 3, page no.416-420, March-2019, Available :http://www.jetir.org/papers/JETIR1903063.pdf

"Study of application of bacteriocin produced by lactic acid bacteria against isolated plant pathogenic bacteria responsible for bacterial wilt in Solanaceous plants", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 3, page no. pp416-420, March-2019, Available at : http://www.jetir.org/papers/JETIR1903063.pdf