• Dr. Reena G. Desai
• Manasvi A. Retiwala

Carbazole is a nitrogen heterocyclic compound released into the environment, used as a chemical feed stock for the production of dyes, medicines and plastics. Carbazole and their derivatives are detected in atmospheric samples, river sediments, marine water and ground water. Enriched Mineral salts medium (MSM ) broth was used to isolate bacteria able to use carbazole as a sole Carbon and Nitrogen and plate assay using MSM agar plate was developed to select carbazole degrading microorganisms. Ten different bacterial isolates were obtained which were capable of utilizing carbazole as the only sole source of carbon and nitrogen added. From the obtained ten isolates C:1, C:2 and C: 3 were from marine water samples, C:4, C:5 and C:6 from marine soil samples, C:7 and C:8 from Pond water samples and C:9, C:10 were from garage soil samples. All isolates were further studied for their “Bioavailability assay” by using different stress and favourable conditions, among all C: 2 isolate showed the maximum considerable and noticeable result in MSM medium. From the bioavailability assay results carbazole degradation studies was carried out using C: 2 isolate by adding 100 ppm and 500 ppm concentration of carbazole in MSM broth medium from which the concentration of carbazole reduced to 8.33 ppm and 50 ppm in 144 hours containing 100 ppm and 500 ppm respectively. Immobilization studies were carried by immobilizing C: 2 isolate in the form of calcium-alginate beads and PVA-alginate beads. Study of Mechanical stability of calcium-alginate and PVA-alginate beads was checked by sonication. PVA-alginate beads were more stable than calcium-alginate beads. Viability of the immobilized cells was tested by calculating colony forming units using both the types of beads, PVA-alginate beads shows more viability than calcium-alginate beads. Carbazole degradation studies with immobilized cells was done by taking absorbance OD600, the concentration reduced to 41.66 ppm and 33.33 ppm in 144 hours using Calcium- alginate beads and PVA- alginate beads respectively containing 100 ppm concentration of carbazole and 241.6 ppm and 120.8 ppm respectively containing 500 ppm concentration of carbazole.

Bacteria, Carbazole, Bioavailability assay, Immobilization, Calcium alginate, Poly vinyl alcohol.

"study of carbazole degrading marine and terrestrial bacterial isolates from valsad district", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 3, page no.818-823, March-2019, Available :http://www.jetir.org/papers/JETIR1903221.pdf

"study of carbazole degrading marine and terrestrial bacterial isolates from valsad district", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 3, page no. pp818-823, March-2019, Available at : http://www.jetir.org/papers/JETIR1903221.pdf