• Ulavayya
• Ashoka Pujar
• Mallikarjun Yadawe

The importance of vinca rosea leaves and flower with different metals as revealed by various literature resources, we planned to carry out synthesis of zirconium nanoparticles using the above extract. Zirconium nanoparticle formations were characterized by UV, FTIR, TEM, TG and XRD. The UV spectra results show a strong resonance centre and surface of zirconium nanoparticles at 299nm. XRD and TEM studies revealed that the synthesized nanoparticles are spherical in shape with average particles size around 0.27nm to 100nm. However, there is limited information about antitumor potential. Therefore, this study focused on determining cytotoxic effects of ZrNPs on MDA-MB-231 breast cancer cells and its mechanism of cell death. The zirconium nanoparticles using an aqueous extract of vinca leaves show potential anticancer activity on MDA-MB-231 breast cancer cells. The toxicity was evaluated using cell viability, metabolic activity, and oxidative stress. MDA-MB-231 breast cancer cells were treated with various concentrations of ZrNPs (5 to 25 μg/mL) for 24 h. We found that ZrNPs inhibited the growth in a dose-dependent manner using MTT assay. ZrNPs showed dose-dependent cytotoxicity against MDA-MB-231 cells through activation of the lactate dehydrogenase (LDH), caspase-3, reactive oxygen species (ROS) generation, eventually leading to induction of apoptosis which was further confirmed through resulting nuclear fragmentation. The present results showed that ZrNPs might be a potential alternative agent for human breast cancer therapy

"BIOMIMETIC SYNTHESIS, CHARACTERIZATION AND ANTICANCER ACTIVITIES OF ZIRCONIUM NANOPARTICLES USING VINCA ROSEA AQUEOUS EXTRACT", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 3, page no.413-420, March-2019, Available :http://www.jetir.org/papers/JETIR1903358.pdf

"BIOMIMETIC SYNTHESIS, CHARACTERIZATION AND ANTICANCER ACTIVITIES OF ZIRCONIUM NANOPARTICLES USING VINCA ROSEA AQUEOUS EXTRACT", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 3, page no. pp413-420, March-2019, Available at : http://www.jetir.org/papers/JETIR1903358.pdf