• M.Vinitha
• G.Prasanna

Present study has been designed to carry out the phytochemical screening, FT-IR analysis, HPTLC finger printing and in vitro antimicrobial activity of aqueous extract of Gmelina asiatica bark. Preliminary phytochemical screening confirmed the presence of carbohydrates, coumarins, glycosides, phytosterols, tannins, phenols etc., FT-IR analysis revealed the presence of functional groups such as alcohol, alkane, fatty acids, alkyne, nitrite, amide, acid, nitro, amine, alkyl halide and ether. HPTLC analysis was performed and the amount of quercetin in ethanolic extract of Gmelina asiatica was found to be 0.0640%w/w. In vitro antimicrobial activity of plant extracts (100,200 and 300 µg/ml) was also performed by using disc diffusion method against the pathogenic bacteria such as Escherichia coli, Bacillus subtilis, Staphylococus aureus, and Pseudomonas aeruginosa, pathogenic fungi such as Candida albicans and Trichoderma viride. Among the three concentrations, 300µg of bark extract was active against both the bacterial and fungal species. Standard antibiotic ampicillin and amphotericin were also subjected for antibacterial and antifungal activities respectively in order to compare the antimicrobial efficacy of plant extract. Based on the present study, bark extract of Gmelina asiatica possess remarkable antimicrobial activity against several pathogens tested and thus can be a good source of natural antimicrobial agent which may be due to the presence of flavonoids like quercetin.

Antimicrobial, Bark extract, FT-IR, Gmelina asiatica, HPTLC, Quercetin Phytochemical.

"PHYTOCHEMICAL SCREENING AND IN VITRO ANTIMICROBIAL ACTIVITY OF Gmelina asiatica L. BARK", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 3, page no.604-616, March-2019, Available :http://www.jetir.org/papers/JETIR1903581.pdf

"PHYTOCHEMICAL SCREENING AND IN VITRO ANTIMICROBIAL ACTIVITY OF Gmelina asiatica L. BARK", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 3, page no. pp604-616, March-2019, Available at : http://www.jetir.org/papers/JETIR1903581.pdf