• Divya Nileshbhai Patel
• Hetal K. Panchal

Biosurfactants are extracellular surface active compounds produced by bacteria, fungi and yeast. Most microbial surfactants are complex molecules, comprising different structures that include lipopeptides, glycolipids, polysaccharides protein complexes, fatty acids and phospholipids. The main criteria used in this study were isolation of biosurfactants producer were hemolytic assay, bacterial adherence to hydrocarbons (BATH), visualization of bacteria in oil droplets, drop collapse test and emulsification assay. Isolation and screening of biosurfactant producing bacteria were performed from marine water samples collected from Tithal beach of Valsad district, Gujarat, India and on nutrient agar plate and 14 isolates were collected. Isolates were characterized and analyzed based on different screening methods like hemolytic assay, growth on mineral salt medium (2% oil as a substrate), BATH assay, visualization of bacteria in oil droplets, drop collapse test and emulsification assay. 10 hemolytic and 4 non-hemolytic isolates were obtained on blood agar plates. Secondary screening on MSM medium were carried out and 6 bacterial isolates were found as potential biosurfactant producers. 4 isolates had shown the red dot of TTC absorption in visualization of bacteria in oil droplets. In drop collapse test 4 isolates using supernatant and cell culture had shown greater activity than deionized water. In Emulsification assay 2 isolates were found promising biosurfactant producer and are selected based on their highest Emulsification activity and were used for production and extraction process. Finally production of biosurfactants was carried out by 2 isolates. The biosurfactant was extracted from the cell- free supernatant by centrifugation. Antibacterial activity of biosurfactants was done against the test organisms but no effect was found on growth. Also the qualitative analysis of the extracted biosurfactant was done by FTIR to check presence of possible functional group.

biosurfactant, hemolysis, BATH assay, Antibacterial activity.

"Production, Extraction and Characterization of Biosurfactant Produced by Marine Bacterial Isolates", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 3, page no.51-59, March-2019, Available :http://www.jetir.org/papers/JETIR1903907.pdf

"Production, Extraction and Characterization of Biosurfactant Produced by Marine Bacterial Isolates", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 3, page no. pp51-59, March-2019, Available at : http://www.jetir.org/papers/JETIR1903907.pdf