• B. Sujatha
• P. Sirisha
• Y. VijayaBharathi

Ragi (Eluesina coracana(L)Gaertn) is the most important small millet in the tropics and is cultivated in more than 25 countries in Africa and South Asia predominantly as a staple food grain. Temperature is one of the most important factors which affect plant growth and its productivity. High temperature stress affects a number of physiological and biochemical processes in plants. According to TIR technique, the seedlings are exposed to optimum induction temperature before being exposed to a severe challenging temperature and subsequently allowed to recovery at room temperature. One resistant genotype (Indaf-8) and one susceptible genotype (CO-7) were selected for the estimation of total protein content and SDS-PAGE analysis. For both selected genotypes, the protein content values of induced seedlings were very high and the values of non-induced seedlings were very low compared to control seedlings. In resistant genotype varied protein banding was observed in the three treatments i.e. control, induced and non-induced. In the induction treatment the total number of bands and the intensity of banding had increased compared to control and non-induction treatments. These new protein bands may be the HSP’s or the enzymes of the anti-oxidant systems which play very important role in providing tolerance against oxidative burst. The induced seedlings had acquired thermotolerance during gradual increasing temperatures and had shown high % survival though they experienced lethal temperature in TIR technique. Hence this method is unique for screening of thermotolerance.

Temperature induction response, acquired thermotolerance, SDS-PAGE, heat shock proteins, anti-oxidants and acclimated.

"Assessment of acquired thermotolerance of Ragi (Eluesina coracana (L.) Gaertn) genotypes by quantitative and qualitative analysis of protein content.", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 3, page no.279-286, March-2019, Available :http://www.jetir.org/papers/JETIR1903B43.pdf

"Assessment of acquired thermotolerance of Ragi (Eluesina coracana (L.) Gaertn) genotypes by quantitative and qualitative analysis of protein content.", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 3, page no. pp279-286, March-2019, Available at : http://www.jetir.org/papers/JETIR1903B43.pdf