• Dr. Chanda Parulekar Berde
• Ms. Pragati V. Shinde

Availability of different pesticides in field provides exposure of several different kinds of microorganisms to pesticides. Most of the organisms die under toxic effect of pesticides but few of them evolve in different ways and use pesticide compounds in metabolism. Several reports are available indicating degradation of different pesticides when they are available in nature in excess. Successful removal of pesticides by the addition of bacteria (bioaugmentation) has been reported earlier for many compounds including chlorpyrifos, endosulfan, parathion, coumphos, ethoprop and atrazine. Chlorpyrifos degrading bacterial isolates were enriched, isolated and purifies from pesticide rich soil. The growth conditions were maintained at 25-30O C. The Gram nature and cell morphology was studied of all isolates. The plate assay for selected isolates was studied by using different concentration of pesticide in medium. Five cultures were selected showing tolerance to 2% chlorpyrifos. These isolates could grow very well in the presence of the pesticide, utilising it for growth. This was demonstrated by tetrazolium chloride test thin layer chromatography.

Pesticides, Organophosphorus, Chlorpyrifos, degradation, bacteria

"Chlorpyrifos degradation by bacterial isolates from pesticide-rich soil", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 3, page no.57-63, March-2019, Available :http://www.jetir.org/papers/JETIR1903E13.pdf

"Chlorpyrifos degradation by bacterial isolates from pesticide-rich soil", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 3, page no. pp57-63, March-2019, Available at : http://www.jetir.org/papers/JETIR1903E13.pdf