• Dr Sabeena

Abstract-Mature Zygotic embryos of Pinus wallichiana were cultured on MS(1/2) and LV(1/2) medium fortified with various Phytohormonal regimes. Embryo explants cultured on various auxin cytokinin combinations produced friable callus at radicle and hypocotyl regions. Multiple shoot proliferation was obtained on MS(1/2) supplemented with BAP(60µM). Axillary shoot regeneration was also observed on LV(1/2) medium enriched with BAP(10µM)+CH(500mg/l). Shoot elongation was observed after transferring the cultures on MS(1/2) basal medium. Rooting of microshoots was obtained on root induction medium containing MS(1/2)+ NAA(0.5µM)+BAP(2.5µM). Rooted Plantlets were transferred to pots and later accilimatized and transferred to green house.

Keywords- Embryo, Pinus wallichiana, multiple shoot, MS medium (Murashige and Skoog,1962), LV medium (Litvay et al.1985), CH (casein hydrolysate)

"Multiple Shoot Formation From Mature Embryos of Pinus wallichiana A.B.Jackson", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 3, page no.635-642, March-2019, Available :http://www.jetir.org/papers/JETIR1903E98.pdf

"Multiple Shoot Formation From Mature Embryos of Pinus wallichiana A.B.Jackson", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 3, page no. pp635-642, March-2019, Available at : http://www.jetir.org/papers/JETIR1903E98.pdf