• Jai Prakash
• Naveen Kumar Arora

Florescent pseudomonads are the efficient and environment-friendly microbes that are widely used for biocontrol and growth promotion in the agricultural crops. In a survey of potential antagonistic stains, a total of 11 fluorescent pseudomonads was isolated from the rhizosphere locale of Stevia rebaudiana. S. rebaudiana is a traditional, ayurvedic herb belonging to the family Asteraceae and famous as a natural high sweetener. Isolate NPSP was identified as a Pseudomonas sp. on the basis of morphological, biochemical and 16s rRNA gene sequencing. Isolate NPSP showed highest chitinase activity (72.66 nmol/min/mg) and siderophore production as compared to other isolates. In addition, NPSP has inhibited the growth of mycelial of Alternaria alternata as observed by dual culture plate method and their inhibition was recorded as 76.6 % after 3 days of incubation period. Thus, using of Pseudomonas sp. as a biocontrol agent could be an economical cheap and eco-friendly method for protection of disease against A. alternata in several agricultural crops including S. rebaudiana and for sustainable agriculture.

Alternaria alternata, Biocontrol agent, Fluorescent Pseudomonads, Stevia rebaudiana

"Study of biocontrol activity of fluorescent pseudomonads against Alternaria alternata, isolated from rhizosphere locale of Stevia rebaudiana ", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 3, page no.632-638, March-2019, Available :http://www.jetir.org/papers/JETIR1903F86.pdf

"Study of biocontrol activity of fluorescent pseudomonads against Alternaria alternata, isolated from rhizosphere locale of Stevia rebaudiana ", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 3, page no. pp632-638, March-2019, Available at : http://www.jetir.org/papers/JETIR1903F86.pdf