• MS. BHOOMI D. PATEL
• DR. ANKIT CHAUDHARY
• SUDHIR GAMI

A simple, rapid, economical, precise and accurate RP-HPLC method for simultaneous estimation of Chlorthalidone, Telmisartan and Benidipine HCl in their Combined Dosage Form has been developed. A reverse phase high performance liquid chromatographic method was developed for the simultaneous estimation of Chlorthalidone, Telmisartan and Benidipine HCl. in Their Combined Dosage Form has been developed. The separation was achieved by C18 (25 cm × 0.46 cm) Hypersil BDS column and Buffer (pH 3.0): Methanol (50:50 v/v) as mobile phase, at a flow rate of 1 ml/min. Detection was carried out at 230 nm. Retention time of Chlorthalidone, Benidipine HCl, and Telmisartan were found to be 4.887 min, 6.690 min and 8.813, respectively. The method has been validated for linearity, accuracy and precision. Linearity observed for Benidipine HCl 2-6 μg/ml, for Telmisartan 20-60 μg/ml and for Chlorthalidone 6.25-18.75 μg/ml. Developed method was found to be accurate, precise and rapid for simultaneous estimation of Chlorthalidone, Telmisartan and Benidipine HCl in their Combined Dosage Form.

Benidipine HCl, Telmisartan, Chlorthalidone, Reverse Phase High Performance Liquid Chromatography Method, Infra-Red Spectroscopy Method, Validation.

"RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF BENIDIPINE HYDROCHLORIDE, TELMISARTAN AND CHLORTHALIDONE IN TABLET", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 3, page no.110-124, March-2019, Available :http://www.jetir.org/papers/JETIR1903I19.pdf

"RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF BENIDIPINE HYDROCHLORIDE, TELMISARTAN AND CHLORTHALIDONE IN TABLET", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 3, page no. pp110-124, March-2019, Available at : http://www.jetir.org/papers/JETIR1903I19.pdf