• B.Gomathi
• K. Dharmar

The present study emphasized on in vitro organogenesis and large scale multiplication protocol for a rare fern Pteris vittata L. The young croziers were harvested and sterilized with 0.05% (w/v) HgCl2 for two minutes and rinsed thrice in sterile distilled water. The sterilized young croziers were cut into 1.0 cm length and cultured on different media augmented with various concentrations and combinations of plant growth regulators. Murashige and Skoog’s (MS) medium added with 2,4-D 1.5 mg/l induced maximum percentage of callus formation. Maximum number (55 ± 1.3) and frequency (70.4 ± 0.72) of shoot formation were observed in MS medium augmented with BAP 1.0 mg/l and IAA 0.5 mg/l. Half strength MS medium augmented with IBA 1.0 mg / l induced maximum number (8.3 ± 0.63) of roots formation with high frequency (80.8 ± 0.33), followed by IAA 1.0 mg/l. The highest percentage of aposporous gametophyte formation was observed on MS medium added with BAP 1.0 mg/l. The micropropagated plants showed maximum percentage (90.6 ± 1.42) establishments during hardening and 52.0 ± 0.9 percentage establishments in the field condition.

Pteris vittata, in vitro, organogenesis, callus, crozier.

"An in vitro study on organogenesis of Pteris vittata L.", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 3, page no.393-396, March-2019, Available :http://www.jetir.org/papers/JETIR1903J57.pdf

"An in vitro study on organogenesis of Pteris vittata L.", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 3, page no. pp393-396, March-2019, Available at : http://www.jetir.org/papers/JETIR1903J57.pdf