• THAKUR PRERNA NANURAM
• Dr. SYED SAHAB AHMAD

The study aimed to find out what affect some of the common heavy metals have on the production of the beta galactosidase enzyme from Fungi. This involved collection of soil samples from different agricultural fields in Maharashtra, India and then isolation of beta galactosidase producing fungi. The beta galactosidase enzyme has a huge market value as it is given to the people who are lactose intolerant. This enzyme hydrolyzes D galactosyl residues from polymers, secondary metabolites as well as oligosaccharides. Most commonly known for breakdown of lactose sugar present in milk that makes it important enzyme in food industry. In nature, beta galactosidase enzyme is found in animals, plants and microbes. But for the commercial production microbes are the best source. Among the different fungal isolates top 3 enzyme producers were chosen for the study. They were cultured under various concentrations of heavy metals Magnesium, Manganese, Chromium, Copper and Cobalt. The enzyme production and activity were calculated for all the three fungal isolates to check their tolerance towards these heavy metals. The findings shows that metal Chromium has maximum toxicity and limiting factor in production of beta galactosidase enzymes from Fungi. The sequence of toxicity is Cr>Co>Mg>Mn>Cu. Thus, for commercial production of this enzyme form fungi one needs to keep note of the heavy metals. The fungal strains that produce useful enzyme life beta galactosidase can also be very useful in the bioremediation of soil that are contaminated with heavy metals to reclaim them for agriculture. In commercial production heavy metals tolerance can help to maintain the production levels. Even this tolerant strains can be used to keep the eye on the increase in concentration of heavy metals in the environment and damaging it.

Magnesium, Manganese, Chromium, Copper and Cobalt, etc...

"STUDY OF PH AND TEMPERATURE ON THE ACTIVITY OF BETA GALACTOSIDASE ENZYME FROM FUNGI", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 3, page no.320-332, March-2019, Available :http://www.jetir.org/papers/JETIR1903M40.pdf

"STUDY OF PH AND TEMPERATURE ON THE ACTIVITY OF BETA GALACTOSIDASE ENZYME FROM FUNGI", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 3, page no. pp320-332, March-2019, Available at : http://www.jetir.org/papers/JETIR1903M40.pdf