• Merugu Madhusudhan
• Dr. Rakesh Kumar Jat
• Dr. Gampa Vijay Kumar

A new method was established for estimation of Nicardipine by RP-HPLC method by using Biorelevent Dissolution Media (FaSSIF). The chromatographic conditions were successfully developed for the separation of Nicardipine by using Kromosil C18 4.5×150 mm 5.0 µm, flow rate was 0.8ml/min, and mobile phase ratio was 65:35% v/v methanol: water, detection wavelength was 265nm. The instrument used was WATERS HPLC Auto Sampler, Separation module 2695, photo diode array detector 996, Empower-software version-2. The retention times were found to be 2.428 mins. The % purity of Nicardipine was found to be 99.87%. The system suitability parameters for Nicardipine such as theoretical plates and tailing factor were found to be4146, 1.23, the. The analytical method was validated according to ICH guidelines (ICH, Q2 (R1)). The linearity study of Nicardipine was found in concentration range of 30µg-150µg and correlation coefficient (r2) was found to be 0.997, % recovery was found to be 100.4%, %RSD for repeatability was 0.5, % RSD for intermediate precision was 1.0. The precision study was precision, robustness and repeatabilty.LOD value was 2.97 and LOQ value was 9.92.Hence the suggested RP-HPLC method can be used for routine analysis of Nicardipine in API and Pharmaceutical dosage form.

Kromosil C18, Nicardipine, RP-HPLC

"ANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF NICARDIPINE BY RP HPLC METHOD", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 3, page no.427-432, March-2019, Available :http://www.jetir.org/papers/JETIR1903N55.pdf

"ANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF NICARDIPINE BY RP HPLC METHOD", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 3, page no. pp427-432, March-2019, Available at : http://www.jetir.org/papers/JETIR1903N55.pdf