• S. Senthamil selvi
• A. Karthikeyan

ABSTRACT: The present study was undertaken to isolate and characterize the hydrocarbon-degrading bacterial isolates from oil-contaminated soil and sludge samples. Totally 17 bacterial isolates were obtained from 35 oil contaminated soil (n=31) and sludge samples (n=4). Among them, six of were finally selected through various screening methods including blue agar plate, hemolysis, drop collapse and oil spreading test using crude oil as a carbon source. From these isolates, the single potential isolate was selected based on the emulsification activity and gravimetric analysis, which was further identified by 16s rRNA gene sequence. The 16S rRNA sequence alignment shows that the isolate 32 was closely related to Pseudomonas stutzeri. Results indicate that this isolate have the potential for future environmental friendly applications such as bioremediation.

Biosurfactant, Pseudomonas stutzeri, blue agar, emulsification index and gravimetric analysis.

"SCREENING AND CHARACTERIZATION OF BIOSURFACTANT-PRODUCING BACTERIA ISOLATED FROM OIL-CONTAMINATED SOIL AND SLUDGE SAMPLES", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 4, page no.467-470, April-2019, Available :http://www.jetir.org/papers/JETIR1904684.pdf

"SCREENING AND CHARACTERIZATION OF BIOSURFACTANT-PRODUCING BACTERIA ISOLATED FROM OIL-CONTAMINATED SOIL AND SLUDGE SAMPLES", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 4, page no. pp467-470, April-2019, Available at : http://www.jetir.org/papers/JETIR1904684.pdf