• N. Sunitha
• A. Bhavani Sailu
• K. Malleswari
• K. Hari Priyanka
• P. Kalyani

The present study was aimed to develop and validate a simple, accurate, precise, reproducible UV-Visible spectrophotometric method for the estimation of Riboflavin present in kiwi fruit. The solvent used in the experiment was methanol and hot water in the ratio 35:65v/v%. Absorption maximum (λmax) of the drug was found to be 370 nm. The Beer’s law was obeyed in the range of 50-500 μg/mL. The method was shown linear in the mentioned concentrations having line equation y = 0.001x+ 0.029 with correlation coefficient r2 of 0.994. The amount of Riboflavin present in kiwi fruit was found to be 0.04mg/ml. The recovery values for Riboflavin present in kiwi fruit ranged from 98.57%-99.16%.The percent relative standard deviation (RSD %) of interday precision was 0.949% and intraday precision was 0.573%. The limit of detection and limit of quantification was 0.079μg/mL and 0.154μg/mL. The percent relative standard deviation of robustness and ruggedness of the method was 0.055-0.239%. Hence, proposed method was precise, accurate and cost effective. This method could be applicable for quantitative determination of the Riboflavin present in kiwi fruit.

kiwi fruit

"Method Development and Validation of Riboflavin Obtained From the Extraction of Kiwi Fruit By UV Spectrophotometry", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 4, page no.460-464, April-2019, Available :http://www.jetir.org/papers/JETIR1904868.pdf

"Method Development and Validation of Riboflavin Obtained From the Extraction of Kiwi Fruit By UV Spectrophotometry", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 4, page no. pp460-464, April-2019, Available at : http://www.jetir.org/papers/JETIR1904868.pdf