• Natarajan.E
• Sankarlal.S
• Rathakrishnan.S

ABSTRACTS The UV-B radiation damaged cells responds to these alterations by either by inducing DNA repair activity or by inducing apoptotic death if the damage is too severe; failure of severely damaged cell to undergo cell death can lead to the formation of an initiator tumor cell. This investigation aimed to test whether extracts from the skin epidermal mucus (SEM) of parrot fish (Scarus ghobban), able to regulate the inflammatory process using the human keratinocyte HaCaT cell line. Cytotoxicity and apoptosis of SEM extracts were determined using MTT assay. Reults showed that HaCaT cells could survive when incubated in SEM at concentrations between 3µg and 10µg/mL. subsequentely, cell viability was compared between cultured HaCat cells exposed to seial dose of UV-B from 1 to 15 mJ/Cm2 and containing 5 different concentrations of SEM(0,3,10, 30,100µ/mL). A increase in cell viability was observed following pretreatment with3 µg and 10µg/mL SEM. Propodium Iodide analysis showed that UV-B irradiated HaCaT cell at 15mJ/Cm2 reduced the amount of live cells when compared to SEM pretreated in HaCaT cells in dose dependent manner. The present study demonstrated that SEM of parrot fish (Scarus ghobban) can attenuate inflammatory proteins triggered by UV-B. Hence, the of parrot fish SEM peptide, may provide new agents for skin anti inflammation, preventing damage due to UV-B.

Keywords: UV-B radiation, Parrot fish, Scarus ghobban, SEM, Cytotoxicity, Apoptosis, Propodium iodide

"ENHANCEMENT OF UV-B RADIATION–MEDIATE APOPTOSIS BY SKIN EPIDERMAL MUCUS OF PARROT FISH IN HaCaT HUMAN IMMORTALISED KERATINOCYTES", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 4, page no.331-340, April-2019, Available :http://www.jetir.org/papers/JETIR1904948.pdf

"ENHANCEMENT OF UV-B RADIATION–MEDIATE APOPTOSIS BY SKIN EPIDERMAL MUCUS OF PARROT FISH IN HaCaT HUMAN IMMORTALISED KERATINOCYTES", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 4, page no. pp331-340, April-2019, Available at : http://www.jetir.org/papers/JETIR1904948.pdf