• CHISTI ZENAB M
• DEDANIA ZARNA
• JAIN VINIT
• DEDANIA RONAK
• PRAJAPATI RICHA

A simple, precise, specific and accurate HPTLC method has been developed and validated for combination of Naproxen Sodium and Esomeprazole Magnesium Trihydrate in pharmaceutical dosage form. The separation was carried out on HPTLC Aluminium plates pre-coated with silica-gel 60 F254(10 ×10 cm) using Ethyl acetate: toluene : methanol (4:5.5:0.5 v/v/v) as mobile phase. HPTLC separation of the two drugs followed by chromatographic measurement was carried out in the absorbance mode at 285 nm. The drugs were resolved satisfactorily with RF values of 0.73 ± 0.01 and 0.43 ± 0.01 for Naproxen Sodium and Esomeprazole Magnesium Trihydrate respectively. The linear regression analysis data for the calibration plots showed good linear relationship with R2=0.999 and 0.998 for Naproxen Sodium and Esomeprazole Magnesium Trihydrate, respectively in the concentration range of 400-2400 ng/spot for Naproxen Sodium and 20-120 ng/spot for Esomeprazole Magnesium Trihydrate. The method was validated for accuracy, precision, specificity and robustness. The limit of detection and quantitation were 2678.72 ng/spot and 811.32 ng/spot, respectively for Naproxen Sodium and 126.00 and 388.68 ng/spot, respectively for Esomeprazole Magnesium Trihydrate. The proposed developed HPTLC method can be applied for identification and quantitative determination of Naproxen Sodium and Esomeprazole Magnesium Trihydrate in bulk drug and drug formulation. Index Terms - HPTLC, Naproxen Sodium, Esomeprazole Magnesium Trihydrate, Pharmaceutical formulations

"HPTLC Method Development and Validation of Naproxen Sodium and Esomeprazole Magnesium Trihydrate in Combined Dosage Form", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 4, page no.317-324, April-2019, Available :http://www.jetir.org/papers/JETIR1904F53.pdf

"HPTLC Method Development and Validation of Naproxen Sodium and Esomeprazole Magnesium Trihydrate in Combined Dosage Form", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 4, page no. pp317-324, April-2019, Available at : http://www.jetir.org/papers/JETIR1904F53.pdf