• Madhusudhana KN
• Satish Kumar Murari
• Surendra DM
• Chandrashekara KT

The current study explains the use of Crotalaria laburnifolia aqueous extract in the synthesis of ZnO nanoparticles and Ag doped ZnO nanoparticles using co-precipitation method. Synthesized Ag doped and undoped ZnO NPs were characterized using powder XRD, Dynamic Light Scattering, Scanning Electron Microscope and Energy Dispersive Spectroscopy. The study assessed the antimicrobial potential of Ag doped ZnO and undoped ZnO NPs against human pathogenic bacteria, Staphylocuccus aureus, Bacillus subtilis, Escherichia coli, P. aeruginosa and Salmonella typhi . The results of the study revealed that the Zn-NPs and Ag doped ZnO showed antibacterial efficacy as well as minimum inhibitory concentration against different human pathogenic bacteria such as B. subtilis, S. aureus, E. coli, P. aeruginosa and S. typhi. The Ag doped ZnO nanoparticles exhibited better antimicrobial activity compared to undoped ZnO and showing activity against all tested microbes except P aeruginosa at 5mM concentration. Resazurin based micro-titre assay showed varied results ranging from 3.25% to 100% inhibition of various microorganism. It also showed that efficacy of antimicrobial activity increased with increase Ag concentration (5mM to 8mM). From the results of the study, it could be concluded that C. laburnifolia successfully caused reduction of Zn and Ag salts to doped and Ag undoped ZnO nanoparticles. Both doped and undoped ZnO caused favorable antimicrobial activity against the tested microbes with Ag doped variant causing better antimicrobial activity. Hence the ZnO and Ag doped ZnO synthesized using C laburnifolia aqueous extract can be further investigated for their toxicity in cells and animals to develop them as potent antimicrobials for biological applications.

Crotolaria laburnifolia, Antibacterial, green synthesis, doping.

"FACILE GREEN SYNTHESIS OF ZnO AND Ag-DOPED ZnO NANOPARTICLES USING CROTALARIA LABURNIFOLIA AQUEOUS EXTRACT FOR ANTIMICROBIAL APPLICATIONS ", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 4, page no.259-265, April-2019, Available :http://www.jetir.org/papers/JETIR1904H48.pdf

"FACILE GREEN SYNTHESIS OF ZnO AND Ag-DOPED ZnO NANOPARTICLES USING CROTALARIA LABURNIFOLIA AQUEOUS EXTRACT FOR ANTIMICROBIAL APPLICATIONS ", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 4, page no. pp259-265, April-2019, Available at : http://www.jetir.org/papers/JETIR1904H48.pdf