• Nair Sreecha Chandran
• Prabha Kumari.C

Biosurfactants are surface active compounds that reduce the interfacial tension between two liquids,or that between a liquid and a solid. Their unique property like non toxic easily biodegradable,eco-friendly and high stability and wide variety of industrial applications makes them highly useful group of compounds.Biosufactants are produced from variety of microorganisms.The objective of the study was to isolate and characterize the biosurfactant producing bacteria from oil contaminated soil and study their effect of biosurfactant production at different physiological parameters like ph, carbon source,nitrogen source,fermentation substrates and metal ions.The isolation and production study was carried out in Minimal salt medium.The isolated strain was identified as Bacillus velezensis using 16S rDNA procedure.The optimal biosurfactant production was analysed by OD at 660nm,which showed the optimal Ph as 9,carbon source as lactose,nitrogen source as peptone, fermentation substrate as kerosene and best metal ion as manganese sulphate.

Biosurfactants, eco-friendly ,microorganism,biodegaradable

"Production and Characterization of biosurfactant from garage soil", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 4, page no.537-541, April-2019, Available :http://www.jetir.org/papers/JETIR1904K78.pdf

"Production and Characterization of biosurfactant from garage soil", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 4, page no. pp537-541, April-2019, Available at : http://www.jetir.org/papers/JETIR1904K78.pdf