• Rakesh V C
• T. Srinivas rao
• Dr. Chandanam Sreedhar
• Akkamma HG
• Josef Yakin

: Chromatography was performed by isocratic reverse phase separation using an Agilent Eclipse plus C18 column of particle size 5µ, (150×4.6mm). The separations were achieved at the UV detection of 276nm using the mobile phase of Phosphate buffer: Methanol in the ratio of 60:40. Flow rate was 1ml/min and the injection volume was set at 20 µl with 10mins of runtime. The retention time was observed at 4.4 mins for Alogliptin benzoate. The method was validated by using various validation parameters like accuracy, precision, linearity, limit of detection (LOD), limit of quantification (LOQ). The standard curve was linear over a working range of 5-30 µg/ml and gave an average correlation factor 0.999. The limit of detection and the limit of quantification were found to be 0.55µg/ml and 1.86µg/ml respectively. The method showed good recoveries and relative standard deviations of intra and inter day assay less than 2. This method can be easily and conveniently used for routine analysis of Alogliptin in bulk and tablet dosage forms

Alogliptin benzoate, RP-HPLC, Accuracy, Precision, Linearity, LOD, LOQ.

"Development and Validation of New RP-HPLC Method for the Determination of Alogliptin Benzoate in Bulk Form and Dosage Form", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 4, page no.52-58, April-2019, Available :http://www.jetir.org/papers/JETIR1904N10.pdf

"Development and Validation of New RP-HPLC Method for the Determination of Alogliptin Benzoate in Bulk Form and Dosage Form", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 4, page no. pp52-58, April-2019, Available at : http://www.jetir.org/papers/JETIR1904N10.pdf