• Mrinalini C. Damle
• Swapnil R. Todkar

The aim of the present study was to develop and validate a stability indicating reverse phase high performance liquid chromatography (RP-HPLC) method for estimation of protocatechuic acid, which is important phytoconstituent of onion peels. An isocratic, RP-HPLC method was developed using HiQ Sil C8 (250×4.6mm) column and using 20mM Potassium dihydrogen phosphate buffer (pH 4): Methanol (30:70 v/v) as mobile phase and detection was carried out at 258 nm. The retention time (RT) of drug was 3.05 ± 0.03 min. The method was validated with respect to linearity, precision, accuracy and robustness. The data of linear regression analysis indicated a good linear relationship over the range of 10-50μg/ml concentrations with a correlation coefficient (R2) of 0.997. Protocatechuic acid was subjected to different stress testing conditions, as per ICH Q1R2 guidelines. The developed method was found to be simple and sensitive for analysis of protocatechuic acid.

Protocatechuic acid, HPLC, Stress testing, onion peels, Validation

"STABILITY INDICATING RP-HPLC METHOD FOR PROTOCATECHUIC ACID, AN ACTIVE PHYTOCONSTITUENT OF ONION PEELS", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 4, page no.91-98, April-2019, Available :http://www.jetir.org/papers/JETIR1904N16.pdf

"STABILITY INDICATING RP-HPLC METHOD FOR PROTOCATECHUIC ACID, AN ACTIVE PHYTOCONSTITUENT OF ONION PEELS", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 4, page no. pp91-98, April-2019, Available at : http://www.jetir.org/papers/JETIR1904N16.pdf