• Sufiyan Ahmad
• . Rageeb Md. Usman1
• Tabrej Mujawar2
• Md. Imran3

Objective: In the present work, A Simple, rapid, sensitive, precise and reproducible specific UV spectrophotometric method for the determination of Sofosbuvir (SOFO) and Ledipasvir (LEDI) in bulk drug and pharmaceutical dosage form were developed and validated. Methods: A simple double beam UV spectrophotometric method has been developed and validated with different parameters such as linearity, precision, repeatability, limit of detection (LOD), Limit of Quantification (LOQ), accuracy as per ICH guidelines. Results: UV-visible Spectrophotometric method, measurement of absorption at maximum wavelength in 10 ml methanol and volume make with water solvent system as reference Sofosbuvir and Ledipasvir were found to be at 250 nm and 333 nm respectively. The drug obeyed the Beer’s law and showed good correlation. Beer’s law was obeyed in concentration range 8-40 µg/ml for Sofosbuvir and 2-10µg/ml for Ledipasvir respectively with correlation coefficient was 0.998. The LOD and LOQ of Sofosbuvir was found to be 0.047 μg/ml and 0.142 μg/ml, Ledipasvir were found to be 1.02 μg/ml and 3.11 μg/ml, respectively. Percentage assay of SOFO and LEDI in tablets. Conclusion: The proposed method is simple, precise, accurate and reproducible can be used for routine analysis of Sofosbuvir and Ledipasvir in bulk and tablet dosage form.

Keywords: Sofosbuvir, Ledipasvir, Method development, Validation, UV spectrophotometric, Dosage forms.

"Development and validation for the simultaneous estimation of Sofosbuvir and Ledipasvir by UV spectrophotometer method in bulk and tablet dosage forms", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 4, page no.1184-1195, April-2019, Available :http://www.jetir.org/papers/JETIR1904R75.pdf

"Development and validation for the simultaneous estimation of Sofosbuvir and Ledipasvir by UV spectrophotometer method in bulk and tablet dosage forms", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 4, page no. pp1184-1195, April-2019, Available at : http://www.jetir.org/papers/JETIR1904R75.pdf