• Tomcy A. T.
• Bineesh.C.P
• Anush N
• Pranav Kumar Prabhakar
• Sajith Chandran

A common problem in clinical laboratory is maintaining stability of biochemical analytes during sample storage. We usually keep samples in a refrigerator or at RT for short durations. Or in a freezer for long durations. Hence time and temperature are two important parameters that affect analysis result. The test result should be accurate and precise for better diagnosis, prognosis and monitoring of disease. The aim of the study was to determine whether the stability of biochemical analytes like glucose, AST, and creatinine is affected by different storage conditions of temperature and time. A set of 30 pooled plasma sample obtained from lithium-heparin tube, each one is aliquoted into 10 different tubes, one from each set is taken for baseline measurement (T1d). The remaining 9 is divided into 3 groups: Group1 Frozen (0 to -8 ℃) Group2 Refrigerated (2 to 8 ℃) & Group3 Room temperature (22 to 27 ℃).30 aliquotes from each group is measured for glucose, AST & creatinine on 4th (T4d), 8th (T8d) & 15th (T15d) day respectively. The mean change were compared with baseline measurement (T1d). The study was performed from 2nd May to 24th July 2019 at Thalassery cooperative hospital. The result obtained shows in sample kept at RT the concentration of glucose keep decreasing whereas creatinine increases and AST shows a variable concentration as the time progress. Statistically significant variation observed in sample kept at room temperature. In short stability of T1d>T4d>T8d>T15d & in group, stability of Group1>Group2>Group3. It was concluded that the parameters are most stable in the immediately separated plasma sample following 15days of storage at freezer, variations shown only in refrigerator and RT sample with the progress of time. It suggests time and temperature mediated mechanism are cause of these changes.

Plasma, Glucose, AST, Creatinine, stability, time, temperature

"Study of Stability of Various Biochemical Analytes in Sample Stored at Different Storage Conditions", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 4, page no.235-242, April-2019, Available :http://www.jetir.org/papers/JETIR1904T35.pdf

"Study of Stability of Various Biochemical Analytes in Sample Stored at Different Storage Conditions", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 4, page no. pp235-242, April-2019, Available at : http://www.jetir.org/papers/JETIR1904T35.pdf