• MUKESH KUMAR
• RAAGEEVA BIMAL

Dalbergia sissoo (Shisham) belongs to sub-family Papilionoidae of the Fabaceae family. It is an important tree for timber, fooder and fuel wood. It is one of the major tree species under social forestry programme, but its mortality is being observed that compel farmers to give up the Shisham plantation. The forest trees are natural gift and divine habitat that makes the country healthy and wealthy. Shisham is an important renewable natural resources. It provides several forest products like fuel, timber, lumber. Paper, fooder etc. Micropropagation has a great potential to improve traditional methods of tree breading. It reduces the time to produce new varieties. Micropropagation is very useful for mass clonal propagation of Dalbergia sissoo or any forest trees. In vitro the rate of multiplication cannot be expected by any of the in vivo methods of clonal propagation. Multiplication cycle is very short. In micropropagation, plant multiplication can continue throughout the year irrespective of the season. It is also feasible to preserve just the germlasm in a deep freeze (cryopreservation) as mass of the cells & later grow a complete plant in tissue culture. So, millions of potential forests trees besides shisham could be stored in a few test tube. The culture leaves showed swelling and good callus formation particularly on media with 2, 4-D and BAP. Stem culture resulted in more callus formation and more organogenesis than leaf culture. Caulogenesis was more common than rhizogenesis and normally occurred through the formed calli. The best media caulogenesis was with 2, 4-D (1.1 mg/I) and BAP (5.5 mg/1). Thus, the in vitro plants were grown in the field. An analysis of characteristics of tissue cultured plants has shown that some characters such as leaf size and colour showed some changings. Thus, the experiments and the morphogenetic observation had shown that the important timber, fodder and fuel wood plant of Fabaceae family Dalbergia sissoo could be micropropagated and improved upon through micropropagation. The ministry of environment and forest constituted the National afforestation and Eco development board (NAEB) in August 1992 for afforestation and management strategies. It has been taken into consideration.

Tree Biotechnology, Social Forestry Programme, Morphogenetic studies, Caulogenesis, Callus, Dalbergia sissoo.

"Advantages of Micro propagation of Tree Biotechnology: An economic and medicinal evaluation", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 4, page no.317-323, April-2019, Available :http://www.jetir.org/papers/JETIR1904T44.pdf

"Advantages of Micro propagation of Tree Biotechnology: An economic and medicinal evaluation", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 4, page no. pp317-323, April-2019, Available at : http://www.jetir.org/papers/JETIR1904T44.pdf