• Ab Rouf Wani
• Satish Mohabe
• Jahangeer Mohd Reshi

Aim: In this research study we evaluated the seeds of Datura stramonium for phytochemical constituents, antibacterial, antifungal and cytotoxic activity. Background: The accumulative evidences suggesting medicinal importance of Datura stramonium coupled with ethnopharmacological importance were the basis to select this plant. Experimental: The ethanolic extraction of dry seeds of Datura stramonium was performed by refluxing with continuous stirring using a magnetic stirrer. The qualitative phytochemical examination of Datura stramonium seeds was executed by carrying out standard tests for carbohydrates, flavonoids, amino acids, saponins, proteins, tannins, starch, alkaloids, terpenoids, reducing sugars, steroids, anthraquinones, and glycosides. The antibacterial (Staphylococcus aureus, Streptococcus pyogenes, Pseudomonas aeruginosa and Escherichia coli) and antifungal (Candida albicans, Aspergillus clavatus and Aspergillus niger) analysis of the extract was performed using disk-diffusion method. The cytotoxic effects of Datura stramonium seeds were examined using MTS proliferation assay. Results: The results of this investigation showed significant phytochemical composition of ethanolic extract of Datura stramonium seeds like the presence of alkaloids, terpenoids, steroids, anthraquinones, carbohydrates and Flavonoids. The ethanolic extract induced significant antibacterial effects against S. aureus, S. pyogenes, P. aeruginosa and E. coli with MIC values of 0.85, 0.97, 0.43 and 0.76 µg/ml, respectively. Further, remarkable antifungal effects were induced by the ethanolic extract against C. albicans, A. clavatus and A. niger with MIC values of 0.30, 0.44 and 0.25 µg/ml, in comparison to that of reference control nystatin (MIC=0.001), respectively. Additionally, strong cytotoxic effects of ethanolic extract of Datura stramonium seeds against Hep-G2 liver carcinoma cells with an IC50 concentration of 50.95 µg/ml. Conclusion: The results showed the presence of medicinally high rated phytochemicals in ethanolic extract of Datura stramonium seeds. The ethanolic extract exhibited remarkable antibacterial and antifungal properties against the selected pathogens. Moreover, the extract exhibited protective effects against liver cancer by inducing cytotoxic effects to Hep-G2 cells

Medicinal plants, Datura stramonium, phytochemicals, antimicrobial, cytotoxicity

"Phytochemical screening, antimicrobial and cytotoxicity of Datura stramonium Seeds growing wild in Kashmir Himalayas, India", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 4, page no.620-630, April-2019, Available :http://www.jetir.org/papers/JETIR1904V38.pdf

"Phytochemical screening, antimicrobial and cytotoxicity of Datura stramonium Seeds growing wild in Kashmir Himalayas, India", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 4, page no. pp620-630, April-2019, Available at : http://www.jetir.org/papers/JETIR1904V38.pdf