• Md Bodruddoza Arefin

Hygrophila schulli (Acanthaceae) a tropical herb, is an important medicinal plant, primarily used as diuretic, haematinic, liver tonic, aphrodisiac and anti-cancer agent. Present work deals with the somatic embryogenesis of the plant. Subculturing of well developed compact calli obtained from cotyledonary leaf and cotyledonary node in the regeneration medium of MS supplemented with BA (1-2 mg/l) singly or in combination with NAA (0.1 mg/l), induced the appearance of large number of embryoids in the form of green tubercles all over their surfaces. 1mg/l BA used singly yielded the best result. Embryoids were initially studied under a stereobinocular microscope and their bipolar nature was subsequently confirmed from Scanning Electron Micrographs. Excised embryoids, grown in basal medium, developed into vigorous plantlets. Hardening and acclimatization were done following standard methods.

Hygrophila schulli, Callus, Regeneration, Embryoid, Somatic-embryo.

"Somatic embryogenesis of Hygrophila schulli (Buch. Ham) M R. & S.M. Almeida", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 4, page no.390-394, April-2019, Available :http://www.jetir.org/papers/JETIR1904W54.pdf

"Somatic embryogenesis of Hygrophila schulli (Buch. Ham) M R. & S.M. Almeida", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 4, page no. pp390-394, April-2019, Available at : http://www.jetir.org/papers/JETIR1904W54.pdf