• Tammanna R. Sahrawat
• Ranbir C. Sobti
• Sukesh C. Sharma
• Uma N. Saikia
• Madan L. Sharma

Objective: A dose-response study to investigate the effect of acute cadmium exposure on oxidative stress and histopathological alterations in liver. Methods: Male Balb/c mice were administered intraperitoneal doses of 0.2, 0.4 and 0.8 mg CdCl2/kg body weight and sacrificed 18 hours post exposure. Oxidative stress was quantified spectrophotometrically by measuring the levels of GSH, total thiols and MDA in liver homogenate. Histology of liver was evaluated under the light and Scanning electron microscope. Results: A dose-response decrease in glutathione and total thiol content with a corresponding increase in MDA levels indicative of lipid peroxidation was observed in the liver of Cd treated mice, as compared to controls. Histopathological alterations were observed only in livers of mice exposed to 0.8 mg CdCl2/kg body weight. Light microscopy showed basophilic depositions, Kupffer cell hyperplasia and lobular inflammation while electron micrographs revealed similar features along with crystalline deposits on hepatocytes, pronounced damage to the endothelial cell fenestrations and bile canalculi. Conclusions: In the present study, it was seen that on acute Cd exposure, with increase in CdCl2 dose there were significant changes in the biochemical parameters indicative of oxidative stress, while a certain amount of Cd load was required for histopathological changes to take place in hepatic tissue.

Cadmium, acute exposure, liver, oxidative stress, scanning electron microscopy, light microscopy.

"HEPATOTOXICITY ON ACUTE EXPOSURE TO CADMIUM CHLORIDE IN MICE", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 5, page no.384-390, May-2019, Available :http://www.jetir.org/papers/JETIR1905B56.pdf

"HEPATOTOXICITY ON ACUTE EXPOSURE TO CADMIUM CHLORIDE IN MICE", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 5, page no. pp384-390, May-2019, Available at : http://www.jetir.org/papers/JETIR1905B56.pdf