• Likhith Gowda M
• Manjunatha H.B

Abstract: Streptococcus mutans is one of the prime bacteria that cause dental caries in humans. The silkworm, Bombyx mori L. larvae infected with S. mutans exhibit characteristic disease symptoms indicating successfull induction of pathogenesis for the first time. However, its impact on biochemical and physiological processes in the host is unknown. Hence, we have planned to uncover the impact of S. mutans infection and antibiotics in the regulation of alanine and aspartate enzyme activity in the silkworm haemolymph. The results revealed that S. mutans infection through haemocoel and oral routes accelerated the activity of Aspartate Aminotransferase (AST) at the rate of 154.37 and 183.33% respectively. Similarly, the activity of Alanine Aminotransferase (ALT) was also found elevated at the rate of 143.15 and 181.73% in comparison with uninfected larvae. Administration of antibiotics – Advent and Taxim through oral route restored the larval health by declining the AAT enzyme activity accounting to 62.66 and 46.69% in the haemolymph. Through haemocoel mode of administration of antibiotics - Advent and Taxim, the highest reduction in AST activity accounting to 20.77 and 16.44% respectively were recorded. Concomitantly, ALT activity in the haemolymph was found to be declined at the rate of 53.07 and 41.63% in the larvae administered with Advent and Taxim antibiotics through the oral route, while 41.55 and 27.70% of reduced enzyme activity was noticed for haemocoel route of administration. Taken together, it was obvious that while S. mutans infection enhanced the AST and ALT activity, the antibiotics declined the activity of these enzymes. So, we propose silkworm as a potent model system and AST and ALT as marker enzymes to evaluate novel drugs against S. mutans and other pathogenic infection.

Alanine aminotranferase, Aspertate aminotransferase, Silkworm, S. mutans

"STREPTOCOCCUS MUTANS INFECTION AND ANTIBIOTIC-MEDIATED VARIATION IN THE ALANINE AMINOTRANSFERASE AND ASPARTATE AMINOTRANSFERASE ACTIVITY IN THE SILKWORM, BOMBYX MORI ", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 5, page no.307-314, May-2019, Available :http://www.jetir.org/papers/JETIR1905C45.pdf

"STREPTOCOCCUS MUTANS INFECTION AND ANTIBIOTIC-MEDIATED VARIATION IN THE ALANINE AMINOTRANSFERASE AND ASPARTATE AMINOTRANSFERASE ACTIVITY IN THE SILKWORM, BOMBYX MORI ", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 5, page no. pp307-314, May-2019, Available at : http://www.jetir.org/papers/JETIR1905C45.pdf