• Shaikh Ayesha
• suparna dugal

The rapidly advancing field of nanomedicine is being explored by researchers as a tool to combat the alarming increase in resistant bacterial infections. Our skin is prone to many challenges like climate change, environmental stress due to which are skin becomes dry and rough, formation of dry callus, dullness etc. Therefore, it is very important to protect our skin from all of the above mentioned problem. The current study employs a novel approach in formulating a skin preparation possessing both antibacterial and anti-oxidant activity. In the present study silver nanoparticles were chemically as well as biologically synthesized (green synthesis) and characterized by UV- visible spectroscopy and the maxima absorption was obtained at 430nm and 470nm respectively. Antimicrobial activity of the synthesized nanoparticles was tested by the agar cup diffusion assay against E.coli and S.aureus as the test organisms. MIC of the chemically synthesized silver nanoparticles was found to be 45.85 ppm whereas, for biologically synthesized silver nanoparticles (Papaya leaf) it was found to be 12.6 ppm. Atomic absorption spectroscopy studies revealed the concentration of silver nanoparticles 50.4ppm, 91.7ppm respectively. Scanning electron microscopy revealed that both chemically as well as biologically synthesized silver nanoparticles were spherical in morphology and approximately 67 nm and 87 nm in diameter respectively. An antibacterial lotion was prepared with the above-mentioned nanoparticles incorporated in it. Further, in order to impart antioxidant activity of the lotion, ascorbic acid was incorporated. Assays performed revealed the lotion to possess good antimicrobial and antioxidant activity.

silver nanoparticles, green synthesis, antibacterial, ascorbic acid, skin lotion.

"The current study CHEMICALLLY AND BIOLOGICALLY SYNTHESIZED SILVER NANOPARTILCES -SYNTHESIS, CHARACTERIZATION AND APPLICATION OF IN A NOVEL SKIN FORMULATIONaimed to develop and characterize silver nanoparticles by chemical and biological synthesis and carry out investigation of their antimicrobial a", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 5, page no.73-79, May-2019, Available :http://www.jetir.org/papers/JETIR1905E10.pdf

"The current study CHEMICALLLY AND BIOLOGICALLY SYNTHESIZED SILVER NANOPARTILCES -SYNTHESIS, CHARACTERIZATION AND APPLICATION OF IN A NOVEL SKIN FORMULATIONaimed to develop and characterize silver nanoparticles by chemical and biological synthesis and carry out investigation of their antimicrobial a", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 5, page no. pp73-79, May-2019, Available at : http://www.jetir.org/papers/JETIR1905E10.pdf