• Tafadzwa Kaurayi
• Lilian R Tomu
• Umang R Solanki
• Vinay M Tripathi
• Karishma Sawardekar

Biosurfactants (BS) are surface active agents produced by yeasts, bacteria and fungi. These biosurfactants are amphiphilic molecules consisting of hydrophilic and hydrophobic domain. In this study, bacterial strains with the potential of producing a biosurfactant were isolated from cheddar cheese obtained from a local supermarket in Goregaon West, Mumbai, India. The bacteria was identified as Lactobacilli species. The ability of isolated strains to produce a biosurfactant was determined by haemolysis test on blood agar. The activity of the biosurfactant obtained from bacteria was tested by standard qualitative methods. The biosurfactant was found to have both emulsifying activity and surface tension reduction. Based on biochemical and TLC tests, the extracted biosurfactant was found to be a lipopeptide. In-vitro analysis of antimicrobial, anti-adhesive andanti-biofilmabilities of the extracted biosurfactant was determined on six biofilm producing uropathogens which include Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis,Pseudomonas aeruginosa, Candida albicans and Staphylococcus species. The extracted biosurfactant displayed anti-adhesive, anti-biofilm and antimicrobial abilities against all the above mentioned microorganisms. Highest anti-adhesive and antimicrobial activities were observed against E. coli, Staphylococcus species, C. albicans and P. aeruginosa however K. pneumoniae and P. mirabilis showed less susceptibility. Together, these capabilities may open up possibilities for biosurfactants as an alternative therapeutic approach for the prevention and treatment of Urinary Tract Infections.

Biofilm, Biosurfactant, anti-biofilm, antimicrobial, uropathogens

"EXTRACTION & CHARACTERIZATION OF A BIOSURFACTANT FROM Lactobacilli speciesAND DETERMINATION OF IT’S ANTI-BIOFILM ACTIVITY ON URINARY TRACT INFECTION (UTI) CAUSING PATHOGENS", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 5, page no.32-42, May-2019, Available :http://www.jetir.org/papers/JETIR1905I05.pdf

"EXTRACTION & CHARACTERIZATION OF A BIOSURFACTANT FROM Lactobacilli speciesAND DETERMINATION OF IT’S ANTI-BIOFILM ACTIVITY ON URINARY TRACT INFECTION (UTI) CAUSING PATHOGENS", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 5, page no. pp32-42, May-2019, Available at : http://www.jetir.org/papers/JETIR1905I05.pdf