• D. Mahajan
• P. Yadav
• P. Mishra
• R. Rane
• N. Colaco

Microalga are currently cultivated commercially for human nutritional products around the world in small to medium scale production systems, with total commercial algal biomass production estimated at about 10,000 tons per year. Microalgae produce vast array of natural products including proteins, enzymes, bioactive compounds and carotenoids. Three genera: Chlorella, Spirulina, and Dunaliella represent 85% of the worldwide production. Pigments and metabolites produced by microalgae are highly common in nature and has high industrial potential. One of the metabolite produced by microalgae is Beta carotene. Carotenoids are known to be crucial for normal vision and have been associated with reducing the risk of several degenerative diseases including cancer. There are more than 400 carotenoids found in nature and beta carotene is perhaps the most important one. In the present investigation, experiment was conducted to study and evaluate the effect of environmental stress for accumulation of Beta-carotene. Main stress factor which was considered for analysis included high salinity as for more carotene production high saline conditions are required. The effect of inorganic compounds i.e. NH4Cl, NaPO4, NaCl on growth and carotene accumulation by halotolerant microalgae was studied. Beta carotene was extracted using solvent extraction method. Maximum Beta carotene accumulation of 2.974mg/L was observed in Bold’s Basal medium supplied with 5% NaCl after 45days of incubation. When grown under different concentration of NaCl (1%, 3%, 9%) and NH3Cl, NaPO4 in BB medium, decreased carotene production was observed. The presence of Beta-Carotene was confirmed using HPLC

Halotolerant microalgae, Beta-Carotene , Salinity, Solvent extraction

"EXTRACTION OF BETA CAROTENE FROM HALO TOLERANT MICROALGAE", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 5, page no.78-84, May-2019, Available :http://www.jetir.org/papers/JETIR1905I11.pdf

"EXTRACTION OF BETA CAROTENE FROM HALO TOLERANT MICROALGAE", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 5, page no. pp78-84, May-2019, Available at : http://www.jetir.org/papers/JETIR1905I11.pdf