• Ramkumar Smita
• Mahida Ankita
• Intwala Siddhi M.

The production of industrial enzymes, including fungi and bacteria has always been the industrial choice due to its large application and feasibility. Microbial lipases are used many industries like food flavouring industry, detergent and as ester and amino acid derivatives, biocatalytic resolution of pharmaceuticals, biosensors in agro chemicals, cosmetics, bioremediation etc. The present study describes the attempt of obtaining potential lipase producing bacteria from soil. 25 isolates were obtained by primary screening and from that 16 isolates were able to produce lipase enzyme. Based on quantity of the lipase produced by all isolates, A31 characterized as Enterobacter sakazaki which could be used as potent microbial source of lipase enzyme. The optimum pH for the maximum lipase production was 9 and optimum temperature was 40º C. Glucose incorporated as carbon source, yeast extract as nitrogen source and Tributyrene as substrate led highest lipase production. The presence of Cd+2 enhanced the lipase production. In the partial purification of lipase by ammonium sulphate precipitation, 30 % pellet gave 84 % yield purification and 70 % pellet gave 55 % yield purification. Key words: Lipase, ammonium sulphate precipitation, pellet, tributyrene

"Isolation, Screening and Characterization of Lipase by Enterobacter sakazaki from farm soil", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 5, page no.368-380, May-2019, Available :http://www.jetir.org/papers/JETIR1905K49.pdf

"Isolation, Screening and Characterization of Lipase by Enterobacter sakazaki from farm soil", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 5, page no. pp368-380, May-2019, Available at : http://www.jetir.org/papers/JETIR1905K49.pdf