• PRIYA C. S.
• DEIVASIGAMANIB
• ANUSHA N
• SHAJAHAN A

A total of 10 chitinolytic streptomycetes were isolated from 40 soil samples collected from completely different elements of Tamil Nadu, India. Among them, a strain selected as DSCH2 [Deivasigamani chennai strain a 2] that made highest polysaccharideolytic activity. In primary and secondary screening in mixture chitin agar was elect and later known as Actinomycete hygroscopicus. In our previous study, the assembly of chitnase was administrated by Actinomycete hygroscopicus VMCH2. The assembly of chitinase by Actinomycete hygroscopicus VMCH2 was optimised victimization completely different growth media, substrate concentration, pH, temperature and period. The utmost polysaccharidease production was ascertained in CCMB (colloidal chitin medium broth) amended 0.1 % mixture polysaccharide at hydrogen ion concentration 7.0 and 35° C when eight days of immunization. Below these optimized growth conditions, Actinomycete hygroscopicus VMCH2 made a complete chitinase activity of 50.05 units /ml as against solely 13.4 units/ml within the initial CCMB production medium, that could be a 4-fold increment. This study is to decrease the period from 8 days to 6 days because the strain is from coastal region.

Streptomyces hygroscopicus, chitinolytic bacteria, chitinase, colloidal chitin, inhibition.

"OPTIMIZATION OF CHITINASE EXTRACTON BY Streptomyces hygroscopicus DSCH 2 ISOLATED FROM THE SAMPLE OF SOIL ", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 5, page no.638-648, May-2019, Available :http://www.jetir.org/papers/JETIR1905M98.pdf

"OPTIMIZATION OF CHITINASE EXTRACTON BY Streptomyces hygroscopicus DSCH 2 ISOLATED FROM THE SAMPLE OF SOIL ", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 5, page no. pp638-648, May-2019, Available at : http://www.jetir.org/papers/JETIR1905M98.pdf