• DR SWAPAN MANDAL

Almost 26-32% of the drugs currently used are still of plant origin. Most of the plants producing these constituents are wild in nature, only a few are cultivated. As the plants have often been collected from the natural flora, there is an increasing depletion of the natural resources. The present study highlights the application of in-vitro culture techniques for rapid multiplication of elite plants through micro propagation of neem. Meristem cultures leading to multiple shoot formation have been exploited in neem (Azadirachta indica). Explants from shoot apices, intermodal meristem cultured on MS supplemented with BA and NAA gave good responses in shoot multiplication. Cassia fistula is another important species of Cassia for anthraquinones. Callus tissue obtained from leaf explants were allowed to grow on MS media supplemented with hormones 2,4-D and kinetin. After successful growth of callus, the tissues were harvested after 60 days. Anthraquinones were extracted by ether and ethyl acetate and subjected to TLC with reference to standard commercial anthraquinone.

Explants, meristem cultures, anthraquinones, in-vitro culture, micropropagation, azadirachtin, pharmaceutic, TLC,

"IN-VITRO STUDIES IN NEEM & CASSIA", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 5, page no.71-74, May-2019, Available :http://www.jetir.org/papers/JETIR1905P09.pdf

"IN-VITRO STUDIES IN NEEM & CASSIA", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 5, page no. pp71-74, May-2019, Available at : http://www.jetir.org/papers/JETIR1905P09.pdf