• Vaishali Sharma
• Nidhi Rana
• Vishal Ahuja
• Ranju Kumari Rathour
• Arvind Kumar Bhatt

Waste management is one of the major challenges to overcome, nowadays and turning waste into energy can be a beneficial option to deal with. Keeping in mind, the study was carried out to utilize agricultural and industrial waste to useful by-products like ethanol and nutraceuticals etc. Agricultural waste contains the lignocellulosic biomass which is nature’s richest energy source i.e. sugars. Cellulase is multi-component enzyme system which hydrolyze cellulose into soluble sugars. Hence in the present investigation 80 cellulase producing microbes were isolated from various sites of Himachal Pradesh. Among them 48 isolates were positive but isolate showing the maximum CMCase and FPCase activity was Bacillus sp.CPS66. The optimization of culture conditions of Bacillus sp. CPS66 resulted into 3.04 fold increase in CMCase and 5.34 in FPCase. Maximum cellulase activity was recorded with 5% inoculum (12 hr old culture) using 2% wheat bran (w/v) as sole carbon source. Among the various nitrogen sources tested, 1% meat extract (w/v) was found as the best nitrogen source for cellulase production. The relative production of enzyme at varied pH (4-11) and temperature ranges (20-55 ˚C) revealed that this enzyme was equally effective over a wide pH range (6 to 9.5) with maximum activity at pH 7.5 and temperature of 40 ˚C. The properties of hyper cellulase producing Bacillus sp.CPS66 will further be utilised for the saccharification of various lignocellulosic wastes for production biofuel.

lignocellulosic biomass, CMCase, FPCase, optimization

"Constitutive Cellulase Production from Wheat Bran Using Bacillus Sp. CPS66 under Submerged Fermentation", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 5, page no.358-365, May-2019, Available :http://www.jetir.org/papers/JETIR1905Q49.pdf

"Constitutive Cellulase Production from Wheat Bran Using Bacillus Sp. CPS66 under Submerged Fermentation", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 5, page no. pp358-365, May-2019, Available at : http://www.jetir.org/papers/JETIR1905Q49.pdf