• Smitha Grace S.R
• Nagashree A R
• Amruthavarshini
• Chaithra Ratnakar
• Dr.Jyoti Bala Chuahan

Abstract: The present study was carried out to determine the phytochemical constituents, thin layer chromatographic profile and UV analysis of Duranta repens flower extracts. The leaves of D.repens were collected, dried, pulverized and extracted with Dichloromethane using maceration method. The extract was concentrated in vacuo with the aid of rotary evaporator to afford a greenish crude dichloromethane extract (DCME). The fractions were subjected to general phytochemical screening and thin-layer chromatography using standard procedures. The fractions were scanned in the wavelength ranging from 200-750nm using Shimadzu 1800 UV –VIS spectrophotometer and characteristics peaks were detected. Qualitative analysis of the phytochemicals revealed the presence of saponins, alkaloids, flavonoids, phenols, steroids and triterpenes. Quantitative analysis showed that the crude flavanoids was the major phytochemical constituent present in highest percentage Thin-layer chromatographic studies using different solvent systems revealed homogenous spots with different Rf values. The UV profile showed different peaks ranging from 220-750nm with different absorptions respectively. These findings provided the evidence that flower of Duranta repens is a potent source for some medicinally important phytochemicals and it justifies its use as a medicinal plant, the antioxidant capacity of of Dr(CH2Cl2)FE was accessed by two different assays: Total Flavanoid and ABTS assay. The flavanoid content increased with increase in the concentration of the extract. The minimum inhibitory concentrations required to scavenge 50% ABTS free radical (IC50) of flower extract was 100µg/ml. This can be further investigated for the isolation and identification of active biochemical compound of medicinal utilities.

Key words: Duranta repens dichloromethane flower extract (Dr(CH2Cl2)FE), TLC, phytochemical screening, ABTS, Flavonoid.

"Phytochemical screening and Antioxidant analysis of flavanoids from flowers of Duranta repens.", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 5, page no.469-473, May-2019, Available :http://www.jetir.org/papers/JETIR1905Q66.pdf

"Phytochemical screening and Antioxidant analysis of flavanoids from flowers of Duranta repens.", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 5, page no. pp469-473, May-2019, Available at : http://www.jetir.org/papers/JETIR1905Q66.pdf