• Saloni Kothari,
• Dr. Neha Tiwari
• Dr.Pragnesh Patani

High performance liquid chromatography (HPLC) serves as an important technique in assessing drug product due to its versatile features like column stability, and capacity to efficiently separate a wide range of compounds with a high detection sensitivity. This review provides information in regards to various stages involved in development and validation of HPLC method. Validation of HPLC method are described in terms of performance characteristics, like accuracy, precision, specificity, linearity, range and limit of detection, limit of quantification, robustness and system suitability testing. All these parameters are used for routine and stability analysis. HPLC method development and validation play an important role in drug discovery, development, and manufacturing of pharmaceutical drugs due to its number of advantages like rapidity, specificity, precision, accuracy and ease of automation. A sequence of events required for HPLC method development and analytical validation are been described in this article.

Accuracy, HPLC, Method development, Precision, Selectivity, Validation.

"A REVIEW ON HPLC METHOD DEVELOPMENT AND VALIDATION", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 5, page no.1195-1203, May-2019, Available :http://www.jetir.org/papers/JETIR1905S79.pdf

"A REVIEW ON HPLC METHOD DEVELOPMENT AND VALIDATION", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 5, page no. pp1195-1203, May-2019, Available at : http://www.jetir.org/papers/JETIR1905S79.pdf