• K.SUNEETHA
• P.HARI CHANDANA

Vitamins are a class of essential nutrients in the body, thus, they play important roles in human health. Thiamine (B1) is essential to the health of all living organisms and deficiency has long been associated with diseases in animals such as fish, bird, alligators, and domesticated ruminant animals. Thiamine deficiency results in the disease called Beri-Beri. Thiamine functions as the co-enzyme thiamine pyrophosphate (TPP) in the metabolism of carbohydrates and branched chain amino acids. Whole grains or foods made from them contain all the essential parts and naturally occurring nutrients of the entire grain seed in their original proportions. The whole grains consists of three main parts: Germ (contains the plant embryo or seed contains genetic material for a new plant, essential fatty acids, vitamin E, B-group vitamins, minerals, phytonutrients), endosperm (provides food for the growing seed starchy carbohydrates and protein), outer bran (contains vitamins, minerals and phytonutrients) along with aleurone layer (located between the endosperm and the bran, but in the milling process, the aleurone layer is removed together with the bran). The thiamine rich whole grains are barley, brown rice, whole wheat. The thiamine was extracted by using boiling water and the identification of thiamine through Thin layer chromatography (TLC). The estimation of thiamine is done through high performance liquid chromatography (HPLC).

Thiamine, HPLC and WHOLE GRAINS

" QUALITATIVE AND QUANTITATVE ESTIMATION OF THIAMINE IN WHOLE GRAINS BY RP – HPLC", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 5, page no.265-279, May 2019, Available :http://www.jetir.org/papers/JETIR1905X33.pdf

" QUALITATIVE AND QUANTITATVE ESTIMATION OF THIAMINE IN WHOLE GRAINS BY RP – HPLC", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 5, page no. pp265-279, May 2019, Available at : http://www.jetir.org/papers/JETIR1905X33.pdf