• Geetha Menon
• Arya Mane

Petroleum hydrocarbon mineralization is of central importance in ecosystem functioning. The mineralization process is transformation of organic compounds into inorganic form by the soil organisms. Microbial population indigenous to petroleum contaminated soil are suitable for such mineralization process that aid bioremediation. The petroleum hydrocarbon utilizing bacterial isolates from the experimental soil samples include Pseudomonas spp, Staphylococcus spp, Bacillus spp, and Corynebacterium spp. These microbial isolates and their consortium of mixed cultures of two, three and four cultures exhibiting mineralization of petroleum residue (1% diesel) was investigated for a period of 60 days. Among the four isolates, ,highest value was obtained for the rate of petroleum residue degradation was with Pseudomonas spp (78%), which also showed highest emulsification rate (60% ). The CO2 evolution of 55 gm kg-1, cell biomass (175 CFU) and 50 % rate of emulsification observed with Consortium 1, a mixed culture of Pseudomonas spp & Staphylococcus spp.;as compared to the other ten consortium studied. Enzyme dehydrogenase activity was observed highest in the inoculum of Staphylococcus spp (84.1UM/min/ml) indicating the potential to degrade petroleum hydrocarbon. The GCMS data also supports our finding that consortium of Pseudomonas spp & Staphylococcus spp are potent to mineralize diesel up to the range C6 to C9 from C19-C44 found in the control samples.

Hydrocarbon Residue, Mineralization, Enzyme, Microbial Consortium

"“ENZYMATIC STUDIES AND MINERALIZATION POTENTIAL OF SOME BACTERIAL ISOLATES FROM SOIL CONTAMINATED WITH PETROLEUM HYDROCARBON RESIDUES”", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 6, page no.351-357, June-2019, Available :http://www.jetir.org/papers/JETIR1906194.pdf

"“ENZYMATIC STUDIES AND MINERALIZATION POTENTIAL OF SOME BACTERIAL ISOLATES FROM SOIL CONTAMINATED WITH PETROLEUM HYDROCARBON RESIDUES”", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 6, page no. pp351-357, June-2019, Available at : http://www.jetir.org/papers/JETIR1906194.pdf