• P. T. S. R. K. Prasada Rao

The present paper describes a simple, accurate and precise reversed phase High Performance Liquid Chromatography (HPLC) method for rapid and simultaneous quantification of Adapalene and Dapsone. The chromatographic separation was achieved on X-Bridge C8 (150x4.6mm, 3.5µ) column. Mobile phase contained a mixture of 1mg Hexane Sulphonic acid of pH=5.0 adjusted with OPA is used as buffer and Acetonitrile in the ratio of 40:60 v/v, flow rate 1.0ml/min and UV detection at 249nm. The proposed method shows a good linearity in the concentration range of 1-15µg/ml of Adapalene and 50-750µg/ml of Dapsone under optimized conditions. Precision and recovery study results are in between 98-102%. In the entire robustness conditions %RSD is below 2.0%. Degradation has minimum effect in stress condition and solutions are stable up to 24hrs. This method is validated for different parameters like Precision, linearity, accuracy, limit of detection (LOD), limit of quantification (LOQ), ruggedness, robustness and forced degradation study were determined according to the ICH Q2B guidelines. All the parameters of validation were found to be within the acceptance range of ICH guidelines.

RP-HPLC, Adapalene and Dapsone

"DEVELOPMENT AND VALIDATION OF ADAPALENE AND DAPSONE IN BULK AND PHARMACEUTICAL DOSAGE FORM BY RP-HPLC AND ITS DEGRADATION", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 6, page no.754-759, June-2019, Available :http://www.jetir.org/papers/JETIR1906253.pdf

"DEVELOPMENT AND VALIDATION OF ADAPALENE AND DAPSONE IN BULK AND PHARMACEUTICAL DOSAGE FORM BY RP-HPLC AND ITS DEGRADATION", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 6, page no. pp754-759, June-2019, Available at : http://www.jetir.org/papers/JETIR1906253.pdf