• M. Manoranjani

An accurate, rapid economical and simple, reliable method was developed and validated for the simultaneous estimation of Netarsudil and Latanoprost using RP-HPLC. In the proposed method efficient chromatographic separation was achieved using X-Bridge Phenyl column (150x4.6mm, 3.5µm) as a stationary phase and acetonitrile: buffer (30:70v/v, 2.5g of Octane-1-Sulphonic acid in 1lt of water adjust pH-2.5 with Ortho Phosphoric Acid) as a mobile phase with a flow rate of 1ml/min and UV detection at 228nm. Chromatography was carried out isocratically at ambient temperature and the run time was approximately 10min. In this method a good linearity was observed in the range of 0.0063-0.0938µg/ml and 0.0013-0.0188µg/ml with the limit of detection (s/n=7, 4) respectively. The proposed method was validated as per ICH guidelines.

Netarsudil, Latanoprost, RP-HPLC

"STABILITY INDICATING ASSAY METHOD DEVELOPMENT AND VALIDATION OF NETARSUDIL AND LATANOPROST BY RP-HPLC AND ITS DEGRADATION", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 6, page no.825-832, June-2019, Available :http://www.jetir.org/papers/JETIR1906262.pdf

"STABILITY INDICATING ASSAY METHOD DEVELOPMENT AND VALIDATION OF NETARSUDIL AND LATANOPROST BY RP-HPLC AND ITS DEGRADATION", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 6, page no. pp825-832, June-2019, Available at : http://www.jetir.org/papers/JETIR1906262.pdf