• Dr. M. David Raju

An accurate, rapid economical and simple, reliable assay method was developed and validated for the simultaneous estimation of Oxymetazoline HCl and Sorbitol using RP-HPLC. In the proposed method efficient chromatographic separation was achieved using Waters X-Terra RP-18 column (150x4.6mm, 3.5µ) as a stationary phase and acetonitrile: 0.1%OPA (70+30), as a mobile phase with a flow rate of 1ml/min and UV detection at 230nm. Chromatography was carried out isocratically at ambient temperature and the run time was approximately 10min. System suitability parameters were studied by injecting the standard six times and results were well under the acceptance criteria. Linearity study was carried out between 10% to 150% levels, R2 value was found to be as 0.999. By using above method assay of marketed formulation was carried out 100.1% was present. Degradation studies of Oxymetazoline HCl and Sorbitol were done, in all conditions purity threshold was more than purity angle and within the acceptable range.

Oxymetazoline HCl, Sorbitol, HPLC

"METHOD DEVELOPMENT AND VALIDATION OF OXYMETAZOLINE HCl AND SORBITOL IN BULK AND PHARMACEUTICAL DOSAGE FORM", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 6, page no.243-250, June-2019, Available :http://www.jetir.org/papers/JETIR1906324.pdf

"METHOD DEVELOPMENT AND VALIDATION OF OXYMETAZOLINE HCl AND SORBITOL IN BULK AND PHARMACEUTICAL DOSAGE FORM", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 6, page no. pp243-250, June-2019, Available at : http://www.jetir.org/papers/JETIR1906324.pdf