• Neha Keshari
• Dr. Cholera MS
• Prof. A. B. Thakar
• Dr. Rahul Gandhi
• Dr. N.N.Bhatt

Amlapitta is the disease which is occure mainly due to unhealthy diet and faulty life style. There are several sign and symptoms appears in Amlapitta some of them are Daha, Amlodgara, Shula, Avipaka, Chhardi etc. In modern it is correlated with Hyperacidity. Amlapitta is found to be a disease of mandagni. In Amlapitta mainly Rasavaha Raktavaha and Annavaha srotas involved. Aims: To carried out study for granular form of Pippalikhanda along with Trivrittavaleha in order to observe stability against microbial contamination. Materials and Methods: Sample of granular form of Pippalikhanda and Trivrittavaleha was studied to check microbial contamination at regular time intervals. Results: From the date of the preparation to the date of last microbiological study at every regular interval sample was subjected to the microbiological study. There was no any contaminations found in microbiological study. Discussion: Thus this study was done to observe the stability of granular form of Pippalikhanda and Trivrittavaleha in order to check Microbial Contamination of the drug prepared and store in suitable climatic conditions and temperature. Thus a baseline Microbial profile was studied at regular interval of 1 month for total 10 month (i.e. time for consumption of prepared drug) and finally at the end of study it was found that there was not presence of any microbial contamination in the drug prepared. Conclusion: In microbiological study of the granular form of Pippalikhanda and Trivrittavaleha, no growth for microorganisms had been found (bacterial or fungal), till 16th Dec 2018 i.e. approximately 10 month from the date of preparation, shows how stable it is along with its good shelf life. Hence in present study the stability test of granular form of Pippalikhanda and Trivrittavaleha with respect to microbiological study was found to be negative at room temperature, dry and humid, warm and cold conditions.

Amlapitta, Hyperacidity, granular form of Pippalikhanda and Trivrittavaleha, microbial contamination.

"Stability study of granular form of Pippalikhanda along with Trivrittavaleha, used in treatment of Amlapitta (Hyperacidity) - with respect to baseline microbial diagnostic modalities", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 6, page no.454-466, June-2019, Available :http://www.jetir.org/papers/JETIR1906650.pdf

"Stability study of granular form of Pippalikhanda along with Trivrittavaleha, used in treatment of Amlapitta (Hyperacidity) - with respect to baseline microbial diagnostic modalities", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 6, page no. pp454-466, June-2019, Available at : http://www.jetir.org/papers/JETIR1906650.pdf