• Dr. Prithviraj Bhandare
• Dr. Tathagat Eknathrao Waghmare
• Dr. G R Naik

The present investigation was carried out during 2013-14 with twenty Neem (Azdirachta indica) ecotypes for protein profiling of seed protein through SDS-PAGE. The profiles of neem ecotypes were studied by extracting the total seed proteins from 20 ecotypes and performed SDS-Polyacrylamide gel electrophoresis. The total seed protein concentration ranged from 10.2-15.4 mg/ml at 660nm and on the basis of banding patterns through SDS-PAGE, indicated that the number of bands found in ecotypes ranged from 08 to 15. The bands on the gel were divided into 2 categories, major and minor bands. The major bands recorded in B1, B2, B3, B5, R2, G1, G2, G3, G4, G5 and Z1 ecotype, less bands recorded in R4, R5, Z3 and Z4 compared to the other varieties while there is no clear band in the B4, R1, R3, Z2 and Z5 ecotype. The total seed protein variation were also analyzed using Un-weighted Pair Group Method with Arithmetic Mean (UPGMA) and resultant cluster analysis based on the data of protein profiling, classified twenty ecotypes into two major groups. Finally the study concluded that, the protein variability analysis clearly showed that there was low heterogeneity because most of the varieties were in the same cluster with respect to seed storage protein.

Azadirachta indica, seed storage protein, protein profile, cluster analysis, SDS PAGE

"IDENTIFICATION OF SEED STORAGE PROTEIN POLYMORPHISM IN AZADIRACHTA INDICA ECOTYPES USING SDS-PAGE", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 6, page no.742-745, June-2019, Available :http://www.jetir.org/papers/JETIR1906882.pdf

"IDENTIFICATION OF SEED STORAGE PROTEIN POLYMORPHISM IN AZADIRACHTA INDICA ECOTYPES USING SDS-PAGE", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 6, page no. pp742-745, June-2019, Available at : http://www.jetir.org/papers/JETIR1906882.pdf