• Dr. Anuruddha Chabukswar
• Ayushi S. Gadekar

A simple, precise and sensitive stability indicating high performance thin layer chromatographic (HPTLC) method has been developed and validated for the analysis of Zidovudine in bulk and in tablet dosage form. The separation was performed on pre-coated silica gel 60 GF254 plates using Ethyl acetate : Methanol (6:4 v/v)as mobile phase. The retention factor (Rf) was found to be 0.43 ± 0.07. The detection of band was carried out at 265nm.The drug was subjected to different stress conditions like acid, base hydrolysis, oxidation, thermal degradation and photolysis. The method was successfully validated according to ICH Q2(R1) guidelines. The linear regression analysis data for the calibration plot showed good linear relationship with R2= 0.9928 in the range of 500-3000ng band-1. The method found to be accurate as results of the recovery studies are close

High performance thin layer chromatography (HPTLC), Zidovudine, method validation, stability indicating method.

"DEVELOPMENT AND VALIDATION OF STABILITY INDICATING HPTLC METHOD FOR ESTIMATION OF ZIDOVUDINE", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 6, page no.43-51, June-2019, Available :http://www.jetir.org/papers/JETIR1906A10.pdf

"DEVELOPMENT AND VALIDATION OF STABILITY INDICATING HPTLC METHOD FOR ESTIMATION OF ZIDOVUDINE", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 6, page no. pp43-51, June-2019, Available at : http://www.jetir.org/papers/JETIR1906A10.pdf